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Alexa Fluor® 488 Armenian hamster Anti-Mouse CD80 Antibody (16-10A1)

Alexa Fluor® 488 Armenian hamster Anti-Mouse CD80 Antibody (16-10A1)

Catalog Number: S0B8178 Application: FCM Reactivity: Ms Conjugation: Alexa Fluor® 488 Brand: Starter
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Product Details

Product Specification


Host Armenian hamster
Synonyms T-lymphocyte activation antigen CD80; Activation B7-1 antigen (B7); B7; Cd80
Location Cell membrane
Accession Q00609
Clone Number 16-10A1
Antibody Type Recombinant mAb
Isotype IgG
Application FCM
Reactivity Ms
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

CD80, also known as B7-1, is a co - stimulatory molecule expressed on antigen - presenting cells (APCs) such as dendritic cells, macrophages and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a co - stimulatory signal necessary for T cell activation, thereby promoting T cell proliferation and differentiation. Additionally, CD80 can also bind to CTLA - 4, an inhibitory receptor on T cells, which acts as a negative regulator of T cell activation. This binding can inhibit the immune response and maintain immune tolerance. Moreover, CD80 - CD28 interaction is involved in the regulation of various immune - related processes, including the formation of immunological synapses and the regulation of cytokine production.

Picture

FC

Flow cytometric analysis of mouse CD80 expression on C57BL/6 mouse splenocytes treated with LPS. C57BL/6 mouse splenocytes (Left) or treated with LPS (500ng/ml, 3d) (Right) were stained with SDT Alexa Fluor® 488 Armenian hamster Anti-Mouse CD80 antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.