2μg (R: reducing condition, N: non-reducing condition).
ADORA2A (with nine mutations) His-Flag-Alfa-Twinstrep Tag Protein, Human (Detergent)
ADORA2A (with nine mutations) His-Flag-Alfa-Twinstrep Tag Protein, Human (Detergent)
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | ADORA2A, adenosine A2a receptor, A2aR, ADORA2, RDC8 |
| Accession | P29274 |
| Concentration | 1.0mg/ml by Nanodrop |
| Amino Acid Sequence | Full length with nine mutations, and Flag tag at N-Terminal, and His-Alfa-Twinstrep tag at C-Terminal |
| Expression System | HEK293 |
| Molecular Weight | 38-45 kDa and 75-95 kDa(Reducing) |
| Purity | ＞90% by SDS-PAGE &＞90% by SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tags & Cleavage sites | 3C |
| Tag | His Tag, Flag Tag, Twinstrep Tag, Alfa Tag |
| Physical Appearance | Liquid with detergent |
| Storage Buffer | 10 mM Hepes, pH 7.5, 100 mM NaCl, 0. 1 % DDM, 0.01 % CHS. |
| Stability & Storage | ·6 months from date of receipt, -60 to -80 °C as supplied. |
| Reference | 1. Lebon G, Warne T, Edwards P C, et al. Agonist-bound adenosine A2A receptor structures reveal common features of GPCR activation[J]. Nature, 2011, 474(7352): 521-525. |
Background
The Adenosine Receptor A2AR (ADORA2A) is a member of the G protein-coupled receptor (GPCR) superfamily. GPCRs are seven-transmembrane proteins that transduce extracellular signals into intracellular responses. A2AR binds adenosine as its endogenous agonist and interacts with Gs and Golf proteins to increase intracellular cAMP levels. This receptor plays crucial roles in cardiac rhythm, cerebral and renal blood flow, immune function, pain modulation, and sleep regulation. It is also associated with pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. The A2AR receptor is a key player in multiple physiological processes and holds significant potential as a therapeutic target. The biotinylated A2AR protein product may enhance drug discovery efforts.
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SDS-PAGE
SEC-HPLC
The purity of ADORA2A (with nine mutations) His-Flag-Alfa-Twinstrep tag Protein, Human (Detergent) is greater than 90% as determined by SEC-HPLC.
