WB result of Goat anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP
Primary antibody: β-tubulin Rabbit mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP
Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Rabbit IgG |
| Immunogen | Rabbit IgG |
| Application | ELISA, WB |
| Reactivity | Rb |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:10000 | |
| ELISA | 1:10000 |
Background
This antibody has been tested by ELISA to ensure minimal cross-reaction with human serum proteins.
Picture
Picture
Western Blot
WB result of Goat anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP
Primary antibody: β-tubulin Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
WB result of Goat anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP
Primary antibody: β-tubulin Rabbit mAb at 1/5000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
