IF shows positive staining in paraffin-embedded human tonsil. Anti-CD45 antibody (S0B0228) was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 594 Conjugate)
Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 594 Conjugate)
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Mouse IgG |
| Antibody Type | Polyclonal antibody |
| Application | IF |
| Reactivity | Ms |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:500 |
Background
This antibody has been tested to ensure minimal cross-reaction with human, Bovine, Horse serum proteins.
Picture
Picture
Immunofluorescence
IF shows positive staining in paraffin-embedded human stomach. Anti-CD45 antibody (S0B0228) was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Goat Anti-Mouse lgG (H+L) (min X Hu, Bov Sr Prot) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
