Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling NA/LE Mouse anti-human CD28 mAb
(S0B0010) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate) at 1/2000 was used as the secondary antibody. Negative control: THP-1
Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate)
Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate)
Price:
Regular price
$310 USD
Regular price
Sale price
$310 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Mouse IgG |
| Antibody Type | Polyclonal antibody |
| Application | ICC, IF |
| Reactivity | Ms |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | Ms |
| IF | 1:500 | Ms |
Background
This antibody has been tested to ensure minimal cross-reaction with human, Bovine, Horse serum proteins.
Picture
Picture
FC
Immunofluorescence
IF shows positive staining in paraffin-embedded human esophagus. Anti- CD44V6 antibody(S0B0614) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti- CD44V6 antibody(S0B0614) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
