WB result of Butyryllysine Recombinant Rabbit mAb
Primary antibody: Butyryllysine Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 30 mM sodium butyrate for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Butyryllysine Recombinant Rabbit mAb (S-R399)
Butyryllysine Recombinant Rabbit mAb (S-R399)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-R399 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 |
Background
Butyrylation of lysine, or the addition of a butyryl group (C4H7O2-) to the ε-amino group of lysine, is a post-translational modification that alters the chemical properties of lysine and potentially impacts the structure and function of proteins. Lysine butyrylation is a relatively new acetylation-like modification that has been found and reported in various biological processes, including energy metabolism, tumor development, and DNA damage repair.
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Western Blot
