Measured in antiviral assays using HepG2 cells infected with vesicular stomatitis virus. The EC50 for this effect is 16 ng/ml.
IL-27 His Tag Protein, Cynomolgus
IL-27 His Tag Protein, Cynomolgus
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Product Details
Product Details
Product Specification
| Species | Cynomolgus |
| Synonyms | Interleukin 27, IL27, IL-27 |
| Amino Acid Sequence | Q14213.2 EBI-3 Arg21-Lys229 |
| Expression System | HEK293 |
| Molecular Weight | 58-62 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Immunity, 16(6), 779-790. |
Background
Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family. It is composed of two subunits, Epstein-Barr virus-induced gene 3 (EBI3) and p28, and signals through a receptor complex formed by WSX-1 (also known as TCCR or IL-27Rα) and gp130.
IL-27 is produced primarily by antigen-presenting cells (APCs), such as monocytes, dendritic cells, and macrophages, upon activation of pattern recognition receptors. The cynomolgus monkey (Macaca fascicularis) IL-27 protein shares a high degree of amino acid identity with its human ortholog (e.g., 92% for the p28 subunit), enabling the translation of findings from preclinical models.
The biological effects of IL-27 are pleiotropic and context-dependent, integrating both pro-inflammatory and anti-inflammatory functions. It plays a key role in the early induction of Th1 differentiation and also exerts immunosuppressive effects. The specific outcome of IL-27 signaling—whether it promotes or resolves inflammation—is determined by the cellular context, disease state, and infection phase.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized IL-27 Protein, Cynomolgus (Cat. No. UA040571) at 2.0μg/mL (100μL/well) can bind IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, Human (Cat. No. UA011319) with EC50 of 25.5-37.6 ng/mL.
