Galectin-1 Protein, Human
Galectin-1 Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | GBP,HBL,HLBP14,HPL,L-14,Lactose-binding lectin 1,Lectin galactoside-binding soluble 1,lectin,galactoside-binding,soluble,1,LGALS1,Putative MAPK-activating protein PM12,S-Lac lectin 1 |
| Amino Acid Sequence | Ala2-Asp135 |
| Expression System | E.coli |
| Molecular Weight | 14-15kDa (Reducing) |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 150mM NaCl, 1mM TCEP, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Galectins are a family of carbohydrate-binding proteins with an affinity for beta-galactosides. Galectin-1 (Gal-1) is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent, with a wide range of biological activity. The intracellular and extracellular activity of Gal-1 has been described. It preferentially binds Laminin, Fibronectin, Galectin-3BP/MAC-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity.
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SDS-PAGE
SPR
Anti-His antibody Immobilized on CM5 Chip captured Human CD45, His tag (Cat. No. S0A1014), can bind Galectin-1 Protein, Human (Cat. No. UA010167) with an affinity constant of 8.99μM as determined in SPR assay.
Anti-His antibody Immobilized on CM5 Chip captured CD45 His Tag Protein, Human (Cat. No. UA010556), can bind Galectin-1 Protein, Human (Cat. No. UA010167) with an affinity constant of 4.78μM as determined in SPR assay.
