2μg（R: reducing conditions, N: non-reducing conditions)
Complement C5a Protein, Mouse
Complement C5a Protein, Mouse
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$736 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Complement Component C5a |
| Accession | P06684 |
| Amino Acid Sequence | Met+Asn679-Arg755 |
| Expression System | E.coli |
| Molecular Weight | 10kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & RP-HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 300mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.5-1mg/ml according to the size in ultrapure water after rapid centrifugation.r rapid centrifugation . |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
Background
Complement Component C5a (C5a) is also known as C5, and is a protein fragment released from complement component C5. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is also chemotactic for dendritic cells (DCs), germinal center B cells, and T cells. Thus, anaphalatoxins participate in the recruitment of various leukocytes into sites of inflammation during infection and tissue injury.
Guidelines
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SDS-PAGE
RP-HPLC
ELISA
Immobilized Complement C5a Protein, Mouse (Cat. No. UA010080) at 2.0μg/mL (100μL/well) can bind Mouse C5a Recombinant mAb (SDT-2892-38) (Cat. No. S0B3690) with EC50 of 0.35-0.40 ng/mL.
