WB result of L-Lactyl Lysine Rabbit pAb
Primary antibody: L-Lactyl Lysine Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
L-Lactyl Lysine Rabbit Polyclonal Antibody
L-Lactyl Lysine Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC, IP |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| ICC | 1:500 |
Background
Lysine lactylation is an emerging post-translational modification that occurs in cellular metabolism, specifically referring to the accumulation of lactic acid modifying lysine residues on histones. Lysine lactylation modifies the chromatin structure, thereby affecting gene expression. Research has found that lactylation sites are widely distributed in gene promoter regions, closely associated with gene transcriptional activation. Lysine lactylation also participates in energy metabolism processes, potentially related to the dynamic balance regulation of histone lysine acetylation.
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Picture
Western Blot
IP
L-Lactyl Lysine Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating L-Lactyl Lysine in 0.4 mg HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate.
Western blot was performed on the immunoprecipitate using L-Lactyl Lysine Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg (Input)
Lane 2: L-Lactyl Lysine Rabbit pAb IP in HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate
Predicted MW: Multiple
Observed MW: Multiple
Immunocytochemistry
ICC analysis of HeLa cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated HeLa cells (below panel). Anti-L-Lactyl Lysine antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
