WB result of Ctip2 Rabbit mAb
Primary antibody: Ctip2 Rabbit mAb at 1/1000 dilution
Lane 1: MOLT-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 115, 125 kDa
Exposure time: 180 s
Ctip2 Recombinant Rabbit mAb (S-R314)
Ctip2 Recombinant Rabbit mAb (S-R314)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Ctip2 |
| Synonyms | BCL11B; Ctip2; RIT1 |
| Location | Nucleus |
| Accession | Q9C0K0 |
| Clone Number | S-R314 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ChIP | 1:20-1:50 |
Background
CTIP2 (also known as BCL11B) is a transcription factor Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. CTIP2 deficiency results in structural striatal defects, impaired spatial learning, and working memory deficits in mice. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Diseases associated with CTIP2 include Immunodeficiency and Intellectual Developmental Disorder with Speech Delay, Dysmorphic Facies, And T-Cell Abnormalities. Among its related pathways are NF-kappaB Signaling and Development and heterogeneity of the ILC family.
Picture
Picture
Western Blot
ChIP
Chromatin immunoprecipitation (ChIP) was performed on MOLT-4 cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Ctip2 Recombinant Rabbit mAb (S-R314) and IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of CDKN1B, MDM2 and SAT-α in
Ctip2 Recombinant Rabbit mAb (S-R314)-immunoprecipitated
sample.
