庆大霉素残留检测试剂盒
庆大霉素残留检测试剂盒
Product Details
Product Details
Product Specification
| Usage |
1. Preparation
4 Sample preparation |
||||||||||||||||||||||||||||||||||||||||||||
| Theory | This kit uses indirect competition ELISA method to determine trace residues of gentamicin in samples. The microwell strip is pre-coated with conjugated antigen, and the residual gentamicin in the sample competes with the conjugated antigen pre-coated on the microwell strip for anti-gentamicin antibody. The enzyme-labeled secondary antibody is added, and then the color is developed by adding TMB substrate. Use a microplate reader to measure the absorbance (OD value) at the wavelength of 450nm/630nm, and the absorbance is negatively correlated with the content of gentamicin in the sample. | ||||||||||||||||||||||||||||||||||||||||||||
| Description | Gentamicin is an alkaline compound extracted from the fermentation broth of the genus Monospore of Actinomyceaceae. It is a commonly used aminoglycoside antibiotic and widely used in culture medium preparation. Clinically, it is mainly used to treat bacterial infections. Gentamicin is neurotoxic, nephrotoxic and ototoxic. Its residues in animal food and biological drugs will affect human health and even cause allergic reactions. European and American countries and China all require its limited use. Gentamicin quantitative detection kit uses indirect competition ELISA Methods, the pre-coated gentamicin antigen on the microwell strip competed with the residual gentamicin in the sample to bind enzyme-labeled gentamicin monoclonal antibody, then the enzyme-labeled secondary antibody was added, and then by adding TMB The substrate developed color, and the absorbance value was detected by a microplate reader. The absorbance value was negatively correlated with the content of gentamicin in the sample. The operation time of this kit is only about one hour, and the linear range is 0.1ng/mL-10ng/mL 。 When doing the linearity verification of gentamicin raw materials, it is recommended to dilute gentamicin raw materials within the linear range of the kit before determination, and dilute them according to a certain proportion to at least 5 The detected concentration is linearly correlated with the dilution factor. Specificity should be verified for other substances present in the process. It is recommended to use high-concentration and low-concentration quality control samples, and add increasing concentrations of relevant interfering substances for specific investigation. The matrix to which no analyte is added should also be measured at the same time. The accuracy of the controls should be within a range and the measured values of the matrix without the addition of analyte should be below the lower limit of quantification. Product Features: Strong specificity: no cross-reaction with penicillin, streptomycin sulfate, kanamycin, ampicillin High sensitivity: the kit sensitivity is 0.1ng/mL Convenient and efficient: using the two-step method, just 1 The results can be obtained in about hours Excellent performance: good calibration curve, high accuracy and good repeatability Product performance indicators: Limit of detection: < 0.1 ng/mL ； Limit of quantification: 0.1 ng/mL Linearity Range: 0.1-10 ng/mL Accuracy (spiked recovery): 70%-130% Accuracy (measurement deviation): ≤15% Repeatability (intra-batch difference): ≤15% Specificity:
|
||||||||||||||||||||||||||||||||||||||||||||
| Composition |
|
||||||||||||||||||||||||||||||||||||||||||||
| General Notes | 1. All components in the kit must be restored to room temperature (20-25 ℃) before use. 2. Mix all components thoroughly before use. The standard product needs to be shortly centrifuged for 5 seconds to concentrate all the liquid on the tube wall and lid at the bottom of the tube; All reagents were returned to 2-8 °C immediately after use. 3. The kit must be used within the validity period, and the corresponding standard curve must be prepared for each test. It is not recommended to use different batches of related reagents in mixed batches. 4. When adding liquid to the microplate, be careful not to touch the bottom of the microplate to prevent damage to the coating layer. Replace the sample loading tank and suction tip in time between different samples and steps to avoid cross-contamination. 5. When patting the slats dry after washing, be careful to prevent the slats from falling off, and be careful not to reuse the sealing film. 6. High concentration may produce black flocs during color development, which is a normal phenomenon, and the slight degree will not affect the final reading result. 7. When reading, pay attention to check whether the detection wavelength and fitting equation are correct. 8. Only by strictly abiding by the operation methods of the instructions and using all the reagents supporting this kit can the best detection effect be guaranteed. 9. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 10. The company is only responsible for the kit itself, and is not responsible for the sample consumption caused by the use of the kit. Users are requested to fully consider the possible usage of samples and reserve sufficient samples before use. 11. The termination solution in this kit is acid solution, so special attention should be paid to operation. 12. Operators should wear personal protective equipment, such as laboratory coats, gloves, masks and goggles. |
||||||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | The kit should be stored at 2-8 ℃, protected from light, and the shelf life is 12 months. Note that the unused kit after opening should still be stored at 2-8 ℃ in the dark. |
