GFR OrganoGel Phenol red free
GFR OrganoGel Phenol red free
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Product Specification
| Usage | See the operating manual for details. |
| Description | The basement membrane is a layer of matrix membrane on the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from basement membrane components extracted from mouse tumor tissue. The main components of the matrix glue are: laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, the matrix glue also contains a variety of growth factors, such as epidermal growth factor EGF, nerve growth factor, (Vukicevic et al. 1992). Product characteristics: This product is liquid at 4°C, but gels when heated to 37°C. After the matrix gum has set, it is placed back at 4° C. overnight and the matrix gum can be liquefied again. |
| Source | mouse tumor basement membrane components |
| General Notes | This matrix begins to solidify at temperatures above 10°C, so it is best to handle the matrix on ice. During organoid passage, in order to avoid enzymes affecting the organoids, the matrix gel can be slowly blown with 4°C pre-cooled basal medium to release the organoids from the matrix gel. |
| Storage Temp. | It is recommended that users repackage the product according to a single dose after the first thaw and store it in a refrigerator at -80°C. It has a shelf life of 2 years. |
| Applications | Suitable for experiments that require color detection (such as colorimetric method, fluorescence method). |
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Immunohistochemistry
Comparison of organoids of colorectal cancer cultured with different brands of matrix gel (Figure 1)
Comparison of organoids of colorectal cancer cultured with different brands of matrix gel (Figure 2)
