1 0

    2D/3D/Organoid ATP Viability Assay Kit

    2D/3D/Organoid ATP Viability Assay Kit

    Catalog Number: abs50059 Brand: Absin
    Share:
    Price:
    $667 USD
    $667 USD
    Size:

    For shipping services or bulk orders, you may request a quotation.
    Secure checkout with
    View full details

    Product Details

    Product Specification

    Usage 1 Preparation of organoids:
    Use suitable for chemiluminescence detection 96 Aperture plate ( The bottom and lid are transparent, but the hole walls are opaque. Recommended product number: abs7242 ), inoculation per well 5ul-10ul Place the organoid matrix gum suspension in the incubator for more than half an hour, and add it after it solidifies. 100ul Organoid medium (e.g., using 384 Well plate, inoculation per well 2.5μl-4μl Organoid matrix gum suspension, the specific dosage depends on the type. 384 (Depending on the well plate), and ensure that the number of cells per well at the time of testing is... 5 Within 10,000 (if using 384 The orifice plate should be controlled at 1 (within 10,000), and at the same time, a matrix gel culture solution well without organoids was set as a negative control, and organoids were cultured according to the conventional method of organoid culture. If necessary, drug treatment organoids may be added. In addition, if necessary, a concentration gradient of the organoid can be set in order to subsequently determine the effect of using the kit.
    2 Reagent preparation:
    1 tube lyophilized powder added 1 tube 10ml buffer Mix well, protect from light, and wait for use.
    Note: Use as soon as possible after dissolution. -80℃ Store away from light. Shelf life is one month.
    （ 1 ) dissolve Organoid ATP The reagent is detected by luminescence, and if necessary, the reagent can be appropriately dispensed.
    （ 2 ) according to 96 orifice plate per orifice 100μl (384 orifice plate per orifice 25μl)   Take the appropriate amount. Organoid ATP  Reagents were detected by luminescence and equilibrated to room temperature.
    3 Organoid viability test:
    （ 1 Remove the organoid culture plate and allow it to equilibrate at room temperature. 10 minute ( It is usually not advisable to exceed 30 minute ) 。
    （ 2 ) will 96 Remove the organoid medium from each well of the well plate and add it to each well. 100μl Organoid ATP luminescence detection reagent (384 orifice plate per orifice 25μl) 。
    Note: There is no need to remove the matrix glue.
    （ 3 Violent oscillation at room temperature (microplate thermostatic oscillator, recommended product number: abs72034 ） 5min The organoids are sufficiently lysed.  
    （ 4 Room temperature ( approx. 25℃) incubation 10 This allows the luminous signal to stabilize for minutes.
    （ 5 Chemiluminescence detection was performed using a multifunctional microplate reader with chemiluminescence detection function. Please set the corresponding parameters according to the instrument requirements. The detection time for each hole is generally... 0.25- 1 Seconds or longer, depending on the instrument's detection sensitivity. Make appropriate adjustments.
    （ 6 The relative viability of the organoid is calculated directly based on chemiluminescence readings, or based on... ATP The standard curve calculated ATP The amount therefore The relative viability of the organoid was calculated.
    Note: Detection results vary depending on the type of organoid. For some... ATP Exceptionally high levels of organoids, when the number of cells reaches 50,000 There may not be a linear correlation after that, but the chemiluminescence reading will still continue to rise.
    Synonym ATP; ATP detection; CTG; ATPlite; luminescence method cell viability detection kit
    Description         This product is specially developed for organoid activity detection. ATP The detection kit has a luminescence technology based on the principle that luciferase uses luciferin and adenosine triphosphate. (ATP) and O2 As substrate, in Mg2+ When present, it can convert chemical energy into light energy. ATP It is both an essential substrate for luciferase-catalyzed luminescence and an energy source for all biological life activities. In a luciferase-catalyzed luminescence reaction, ATP Within a certain concentration range, its concentration is related to... The light intensity has a linear relationship.
            This kit adopts bioluminescence. (bioluminescent) Law, utilization Firefly luciferase( firefly luciferase ) catalytic substrate —— Conversion and efficient utilization of fluorescein ATP The energy emits photons. Luminous signals and existing ATP The quantity is directly proportional, and ATP directly proportional to the number of cells present in the organoid.

            This kit is designed for multi-well plates for automated high-throughput screening. (HTS) , an ideal choice for organoid proliferation and toxicity analysis. The homogenization test step is to add a single reagent directly to the well plate containing the organoid medium without removing the matrix gel.
            homogeneous detection " sample addition - mixing - detection " The operating protocol allows organoid lysis and the resulting luminescent signal to be related to the existing... ATP The quantity is directly proportional, and ATP The amount is directly proportional to the number of cells in the organoid. The unique homogeneous testing protocol avoids those Requires multiple steps ATP Errors that may be introduced by the detection method.
    Product Composition:
    components 10mL 100mL preservation method
    Organoid ATP fluorescent dye 1 branch 10 branch 2-8℃ Protect from light
    Organoid ATP dye buffer 10mL 10×10mL 2-8℃ Protect from light
    Note: This product is recommended for immediate use.
    Product Features:
    （ 1 This simplifies the organoid activity detection steps: homogeneous. " sample addition - mixing - detection " The protocol reduces the operational steps required for other similar tests.
    （ 2 Fewer organoids are used: Cell numbers below the lower detection limits of commonly used colorimetric and fluorescent methods can be accurately detected. Reduces the number of cells required for each detection reaction.
    （ 3 Results were obtained quickly: after adding reagents. 10 Data can be obtained in minutes.
    （ 4 You can choose your own assay protocol: it can be used for multiple types of multi-well plate operations. A luminescence detector can be used or CCD The imaging device records the data.
    （ 5 The culture plate can be continuously processed: the luminescence signal is stable, and the sample can be processed in batches.
    General Notes 1. The activity of luciferase is relatively sensitive to temperature, so both organoids and detection reagents need to be equilibrated to room temperature before the reaction. Please mix the test reagents well before use.
    2. The detection reagents in this kit contain luciferase, and repeated freezing and thawing will gradually inactivate it. To achieve good results, it can be appropriately repackaged and stored after the first dissolution, but it is important to note that the repackaged containers must not be contaminated with ATP.
    3. When the solvent content of the drug to be tested is high, it may interfere with the luciferase reaction, thereby affecting the chemiluminescence signal. Solvent interference can be eliminated by providing organoid broth control wells containing solvent.
    4. White or black 96-well or 384-well plates suitable for organoid culture must be used for testing. If you use a normal clear 96-well plate or 384-well plate, adjacent wells will interfere with each other.
    5. This product is for scientific research purposes only by professionals and may not be used for clinical diagnosis or treatment, for food or medicine, or for storage in ordinary residences.
    6. For your safety and health, please wear a lab coat and disposable gloves.
    Storage Temp. 2-8℃, protected from light, shelf life 12 months.