WB result of S-RMabMix™ Succinyllysine Rabbit mAb
Primary antibody: S-RMabMix™ Succinyllysine Rabbit mAb at 1/500 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
S-RMabMixTM Succinyllysine Rabbit mAb
S-RMabMixTM Succinyllysine Rabbit mAb
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$160 USD
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$160 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Succinyllysine |
| Synonyms | Ksucc, succinylation |
| Immunogen | Synthetic Peptide |
| Antibody Type | Recombinant mAb Mix |
| Isotype | IgG |
| Application | WB |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:500 | Ms |
Background
Succinyllysine is a form of post-translational modification (PTM) involving the attachment of a succinyl group to the lysine residues in proteins. This modification, which is reversible and dynamic, has been identified in both prokaryotic and eukaryotic cells and is involved in the regulation of various cellular and biological processes. Succinyllysine is implicated in altering enzyme activities of metabolic enzymes and controlling protein-protein or protein-nucleic acid interactions, which can have significant effects on cellular functions such as replication, transcription, and are associated with various diseases including cardiovascular diseases and cancer. The modification is suggested to be regulated by enzymes like sirtuins and is responsive to different physiological conditions, indicating its importance in cellular metabolism and disease development.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of S-RMabMix™ Succinyllysine Rabbit mAb
Lane 1: Modified peptide library (Succinyl lysine)
Lane 2: Unmodified peptide library control
Lane 3: Modified peptide library (Itaconyl lysine)
Lane 4: Modified peptide library (Malonyl lysine)
Lane 5: Modified peptide library (Fumaryl lysine)
Lane 6: Modified peptide library (Crotonyl lysine)
Lane 7: Modified peptide library (Butyryl lysine)
Lane 8: Modified peptide library (L-lactyl lysine)
Lane 9: Modified peptide library (Propionyl lysine)
Lane 10: Modified peptide library (Actyl lysine)
Primary antibody: S-RMabMix™ Succinyllysine Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
