Thyroglobulin Recombinant Rabbit mAb (SDT-209-297)

Catalog Number: S0B2156 Application: IHC-P,IF Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl 500μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Thyroglobulin
Synonyms	Tg, TG
Immunogen	Native protein
Location	Secreted
Accession	P01266
Clone Number	SDT-209-297
Antibody Type	Rabbit mAb
Application	IHC-P, IF
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.125 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:1000	null
IF	1:125	null

Background

Thyroglobulin is a large protein used by the thyroid gland to make thyroid hormones (T4 and T3) and store iodine in the body, made in the thyroid by the so-called follicular cells. When the thyroid is stimulated by thyroid-stimulating hormone (TSH), it combines iodine with thyroglobulin to create the hormones T4 and T3. While most thyroglobulin stays in the thyroid, a small amount leaks out into the bloodstream. Levels in the blood are directly proportional to the size of the thyroid. For example, thyroid cancer cells enlarge the gland and make thyroglobulin in high amounts. Any disorder that increases the size of the thyroid (cancer, autoimmune disease, nodules, etc.) can raise thyroglobulin levels.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human thyroid. Anti-Thyroglobulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-Thyroglobulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse thyroid. Anti-Thyroglobulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Anti-Thyroglobulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat thyroid. Anti-Thyroglobulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-Thyroglobulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

Negative control:IF shows negative staining in paraffin-embedded human cardiac muscle. Anti-Thyroglobulin antibody was used at 1/125 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.