IHC shows positive staining in paraffin-embedded human ×××. Anti-SDF1/CXCL12 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
SDF1/CXCL12 Recombinant Rabbit mAb, PBS Only (SDT-3675)
SDF1/CXCL12 Recombinant Rabbit mAb, PBS Only (SDT-3675)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SDF1/CXCL12 |
| Synonyms | Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas (IRH; hIRH); Pre-B cell growth-stimulating factor (PBSF); SDF1A; SDF1B |
| Location | Secreted |
| Accession | P48061 |
| Clone Number | SDT-3675 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250 | Hu |
Background
Stromal cell-derived factor-1 (SDF-1), also designated CXCL12, is a 67-amino-acid constitutively secreted chemokine that signals exclusively through the Gαi-protein-coupled receptor CXCR4 (and atypical CXCR7) to orchestrate embryonic development, adult stem-cell homing, immune surveillance, vascular remodeling, and neural circuitry; its 3-stranded β-sheet flanked by an α-helix and disulfide-stabilized loops binds CXCR4 with nanomolar affinity, triggering PI3K–Akt, MAPK/ERK, and calcium flux that mediate chemotaxis, survival, and proliferation, whereas dysregulated SDF-1/CXCR4 signaling fuels cancer metastasis, inflammatory disease, and HIV entry, making the pathway a prime target for small-molecule antagonists, peptide analogs, and neutralizing antibodies in oncology and regenerative medicine.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SDF1/CXCL12 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SDF1/CXCL12 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
