IHC shows positive staining in paraffin-embedded human spleen. Anti-PF4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
PF4 Recombinant Rabbit mAb (SDT-4218)
PF4 Recombinant Rabbit mAb (SDT-4218)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PF4 |
| Synonyms | Platelet factor 4; PF-4; C-X-C motif chemokine 4; Iroplact; Oncostatin-A; CXCL4; SCYB4 |
| Location | Secreted |
| Accession | P02776 |
| Clone Number | SDT-4218 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
Platelet factor 4 (PF4) is a 70–amino-acid, 7.8-kDa CXC chemokine stored in platelet α-granules and released upon activation, where it binds glycosaminoglycans such as heparin with ultra-high affinity, neutralizes the anticoagulant activity of heparin by bridging and clustering it on platelet or endothelial surfaces, and forms stable tetramers that can subsequently assemble into ultra-large immune complexes recognized by anti-PF4/heparin antibodies in heparin-induced thrombocytopenia (HIT), while also exerting broader immune-modulatory effects by recruiting and activating leukocytes, promoting neutrophil extracellular trap (NET) formation, enhancing monocyte adhesion and phagocytosis, and amplifying thrombo-inflammation in conditions such as sepsis, COVID-19, and atherosclerosis through interaction with receptors including CXCR3, TLR4, and FcγRIIA.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-PF4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PF4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
