WB result of PAX6 Recombinant Rabbit mAb
Primary antibody: PAX6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
PAX6 Recombinant Rabbit mAb (SDT-1258-145)
PAX6 Recombinant Rabbit mAb (SDT-1258-145)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PAX6 |
| Synonyms | Paired box protein Pax-6; Aniridia type II protein; Oculorhombin; AN2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P26367 |
| Clone Number | SDT-1258-145 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | 293T, Neuro-2a |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
PAX6, also known as Paired Box 6, is a highly conserved transcription factor that plays a crucial role in the development of multiple organs and tissues, particularly the eyes, brain, and pancreas. It is involved in the regulation of gene expression during embryonic development and is essential for the formation of the eyes, central nervous system, and endocrine pancreas. Mutations in the PAX6 gene can lead to a variety of conditions, including aniridia (absence of the iris), microphthalmia (small eyes), and coloboma (gaps in eye structures). Additionally, PAX6 is implicated in the development of the olfactory bulb and the regulation of neuronal migration and cell proliferation. Its importance is further highlighted by its role in maintaining the normal function of certain cells after birth.
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Western Blot
WB result of PAX6 Recombinant Rabbit mAb
Primary antibody: PAX6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human retina. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse retina. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat retina. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
