WB result of MUC5AC Recombinant Rabbit mAb
Primary antibody: MUC5AC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 585 kDa
Observed MW: 130-600 kDa
MUC5AC Recombinant Rabbit mAb, PBS Only (SDT-2630-23)
MUC5AC Recombinant Rabbit mAb, PBS Only (SDT-2630-23)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MUC5AC |
| Synonyms | Mucin-5AC; MUC-5AC Curated; Gastric mucin; Major airway glycoprotein; Mucin-5 subtype AC; tracheobronchial; Tracheobronchial mucin (TBM); MUC5 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P98088 |
| Clone Number | SDT-2630-23 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu |
| Positive Sample | A549 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000-1:2000 | Hu |
| ICC | 1:500 | Hu |
| IF | 1:500 | Hu |
Background
MUC5AC is a gel-forming mucin glycoprotein secreted by goblet cells of the respiratory and gastrointestinal tracts, forming a viscous layer that protects epithelial surfaces by trapping inhaled pathogens and particulates, and its overexpression is a hallmark of mucus hypersecretion diseases such as asthma, COPD and cystic fibrosis.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A549 cells (top panel) and negative staining in HeLa cells (below panel). Anti-MUC5AC antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded human stomach. Anti-MUC5AC antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
