WB result of LICAM Recombinant Rabbit mAb
Primary antibody: LICAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Negative control: U-87 MG whole cell lysate; LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 140 kDa
Observed MW: 280 kDa
LICAM Recombinant Rabbit mAb, PBS Only (SDT-2594-39)
LICAM Recombinant Rabbit mAb, PBS Only (SDT-2594-39)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LICAM |
| Synonyms | Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; CAML1; MIC5; L1CAM |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P32004 |
| Clone Number | SDT-2594-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution,4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu |
| IHC-P | 1:250 | Hu |
Background
LICAM (L1 cell adhesion molecule) is a 200–220 kDa transmembrane glycoprotein of the immunoglobulin superfamily that functions as a pivotal regulator of neural development—guiding neuronal migration, axon outgrowth and fasciculation via homophilic and heterophilic interactions with integrins and other adhesion receptors—and, when re-expressed in cancers such as glioblastoma, lung, endometrial and renal carcinomas, is proteolytically cleaved into soluble ectodomain and cytoplasmic fragments that activate integrin–FAK–GRB2–SOS–RAS signaling, thereby enhancing tumor-cell motility, invasion, trans-endothelial migration and metastasis and serving as an independent marker of poor prognosis and a potential therapeutic target.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
