HNF4α Recombinant Rabbit mAb (SDT-373-64)

Catalog Number: S0B2172 Application: WB,IHC-P,ICC,FCM,IP Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

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$131.00 SGD

Size: 25μl 100 μl 500μl

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Product Details

Product Specification

Host	Rabbit
Antigen	HNF4α
Synonyms	Hepatocyte nuclear factor 4, NR2A1, HNF-4-alpha, HNF-4, TCF-14
Immunogen	Recombinant Protein
Location	Nucleus
Accession	P49698
Clone Number	SDT-373-64
Antibody Type	Recombinant mAb
Application	WB, IHC-P, ICC, IP, ICFCM
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000
IHC-P	1:500
ICC	1:500
IP	1:50
ICFCM	1:500

Background

HNF4α is a transcription factor that plays an important role in liver morphogenesis and maintenance of proper hepatocyte function in a mature liver. Most liver diseases have been associated with altered HNF4α expression, isoform ratios, and localization. Therefore, many studies have indicated that HNF4α may potentially be a target gene for the regression of fibrosis and cirrhosis [PMID: 35114889].

Picture

Western Blot

WB result of HNF4α Rabbit mAb Primary antibody: HNF4α Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Lane 3: SW480 whole cell lysate 20 µg Lane 4: Caco-2 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 53kDa Observed MW: 45~55 kDa

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Left) / HepG2 (Right) cells labelling HNF4α antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: HeLa

IP

HNF4α Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating HNF4α in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using HNF4α Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: HNF4α Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 53 kDa
Observed MW: 45~55 kDa
Exposure time: 20 s

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-HNF4α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HepG2 cells. Anti-HNF4α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

Negative control: ICC shows negative staining in HeLa cells. Anti-HNF4α antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).