WB result of Fumarate hydratase Recombinant Rabbit mAb
Primary antibody: Fumarate hydratase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 46 kDa
Fumarate hydratase Recombinant Rabbit mAb (SDT-2434-54)
Fumarate hydratase Recombinant Rabbit mAb (SDT-2434-54)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Fumarate hydratase |
| Synonyms | Fumarate hydratase, mitochondrial; Fumarase; HsFH; FH |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | P07954 |
| Clone Number | SDT-2434-54 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, HepG2, MCF7, NIH/3T3, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Fumarate hydratase (FH) is a mitochondrial and cytoplasmic metabolic enzyme encoded by the FH gene that catalyzes the reversible stereospecific hydration of fumarate to L-malate within the tricarboxylic acid cycle, a reaction essential for NADH production and cellular energy homeostasis; beyond this canonical metabolic role, FH acts as a tumor suppressor because loss-of-function mutations lead to intracellular accumulation of the oncometabolite fumarate, which inhibits α-ketoglutarate-dependent dioxygenases (including HIF prolyl-hydroxylases and histone demethylases), thereby stabilizing HIF-1α, driving pseudohypoxic gene expression, succinating KEAP1 to activate Nrf2 antioxidant signaling, and ultimately promoting hereditary leiomyomatosis and renal cell carcinoma, while complete FH deficiency causes the severe neurometabolic disorder fumaric aciduria characterized by encephalopathy, developmental delay, and elevated fumarate levels, and the enzyme also moonlights in DNA repair by translocating to double-strand breaks where locally generated fumarate enhances non-homologous end-joining through inhibition of KDM2B and increased H3K36 dimethylation.
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Western Blot
WB result of Fumarate hydratase Recombinant Rabbit mAb
Primary antibody: Fumarate hydratase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 46 kDa
WB result of Fumarate hydratase Recombinant Rabbit mAb
Primary antibody: Fumarate hydratase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 46 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Fumarate hydratase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
