WB result of FGFR2b Recombinant Rabbit mAb
Primary antibody: FGFR2b Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW620 whole cell lysate 20 µg
Lane 2: KATO III whole cell lysate 20 µg
Negative control: SW620 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa
Observed MW: 90~145 kDa
FGFR2b Recombinant Rabbit mAb (SDT-423-121)
FGFR2b Recombinant Rabbit mAb (SDT-423-121)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FGFR2b |
| Synonyms | FGFR2IIIb; Fibroblast growth factor receptor 2b; Fibroblast growth factor receptor 2IIIb |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P21802-3 |
| Clone Number | SDT-423-121 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | KATO III |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
FGFR2b is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR2b has important roles in embryonic development and tissue repair, especially bone and blood vessels. Recent studies have found that about 30% of patients overexpress FGFR2b protein, which may be used as a new biomarker and research target for gastric cancer.
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Picture
Western Blot
WB result of FGFR2b Recombinant Rabbit mAb
Primary antibody: FGFR2b Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: recombinant human FGFR2b protein 10 ng (CAT. NO. UA010145)
Lane 2: recombinant human FGFR2c protein 10 ng (UniProt: P21802-1)
Lane 3: recombinant human FGFR1 protein 10 ng (CAT. NO. UA020008)
Lane 4: recombinant human FGFR3 protein 10 ng
Lane 5: recombinant human FGFR4 protein 10 ng (CAT. NO. UA020009)
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa
Observed MW: 38~60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human gastric cancer (case 1). Anti- FGFR2b antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human gastric cancer (case 2). Anti- FGFR2b antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
