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ERCC1 Recombinant Rabbit mAb,PBS Only (SDT-2458-29)

ERCC1 Recombinant Rabbit mAb,PBS Only (SDT-2458-29)

Catalog Number: S0B2437P Application: WB, IHC-P, ICC Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen ERCC1
Synonyms DNA excision repair protein ERCC-1
Immunogen Recombinant Protein
Location Cytoplasm, Nucleus
Accession P07992
Clone Number SDT-2458-29
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P, ICC
Reactivity Hu
Positive Sample HeLa, HepG2, SK-BR-3, MCF7, T-47D
Purification Protein A
Concentration 1 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS
Stability & Storage

12 months from date of receipt / reconstitution, 4 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:250 Hu
ICC 1:2000 Hu

Background

ERCC1 (Excision Repair Cross-Complementation Group 1) is a 297-amino-acid protein that, together with its obligate partner XPF (ERCC4), forms a structure-specific heterodimeric endonuclease essential for nucleotide excision repair (NER) of helix-distorting DNA lesions caused by UV light or bulky adducts and for interstrand crosslink repair; the ERCC1–XPF complex incises the damaged DNA strand on the 5′ side of the lesion, enabling excision of a ~24–32-nt oligonucleotide, and its expression level or polymorphisms (e.g., C118T, C8092A) are extensively studied as predictive biomarkers for resistance to platinum-based chemotherapy in lung, ovarian, and other solid tumors because reduced ERCC1-mediated DNA repair enhances platinum-induced cytotoxicity.

Picture

Western Blot

WB result of ERCC1 Recombinant Rabbit mAb
Primary antibody: ERCC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in SK-BR-3 cells. Anti-ERCC1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).