WB result of Desmocollin-2/3(DSC2/3) Recombinant Rabbit mAb
Primary antibody: Desmocollin-2/3(DSC2/3) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 100 kDa
Observed MW: 115 kDa
Desmocollin-2/3(DSC2/3) Recombinant Rabbit mAb (SDT-2102-59)
Desmocollin-2/3(DSC2/3) Recombinant Rabbit mAb (SDT-2102-59)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Desmocollin-2/3(DSC2/3) |
| Synonyms | Cadherin family member 2; Desmosomal glycoprotein II; Desmosomal glycoprotein III; CDHF2 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q02487 |
| Clone Number | SDT-2102-59 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, A431, PC-3, HepG2, human tonsil, human cardiac muscle, human prostate, human esophagus, human oral squamous cell carcinoma, human head and neck squamous cell carcinoma |
| Predicted Reactivity | / |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
Desmocollin-2/3 (DSC2/3) are transmembrane proteins belonging to the calcium-dependent cadherin superfamily and are essential components of desmosomes, which are intercellular junctions that provide structural integrity to epithelial tissues. These proteins mediate cell-cell adhesion through homophilic and heterophilic interactions with other desmosomal cadherins, such as desmogleins. DSC2/3 play crucial roles in maintaining tissue cohesion, especially in mechanically stressed environments. Abnormal expression or dysfunction of DSC2/3 has been implicated in various diseases, including skin blistering disorders like pemphigus and arrhythmogenic cardiomyopathy. In cancer, DSC2/3 can have dual roles, acting as tumor suppressors or promoters depending on the context. For example, DSC2 is downregulated in many cancers, such as hepatocellular carcinoma and gastric cancer, where it inhibits cell proliferation and promotes apoptosis. Conversely, in some cancers like pancreatic cancer, loss of DSC2/3 may lead to increased cell migration and metastasis.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Desmocollin-2/3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Desmocollin-2/3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human esophagus. Anti-Desmocollin-2/3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human oral squamous cell carcinoma. Anti-Desmocollin-2/3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human head and neck squamous cell carcinoma. Anti-Desmocollin-2/3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression Atlas
Expression of Desmocollin-2/3 in human tissue.
Expression of Desmocollin-2/3 in tumor tissue.
