IHC shows positive staining in paraffin-embedded human prostate. Anti-CK8/18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CK8/18 Recombinant Rabbit mAb (SDT-P005)
CK8/18 Recombinant Rabbit mAb (SDT-P005)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CK8/18 |
| Synonyms | Cytokeratin 8/18, K8/18 |
| Location | Cytoplasm |
| Clone Number | SDT-P005 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.28 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:1000 | null |
| IF | 1:1000 | null |
Background
CK 8/18 (Cytokeratin 8/18) is expressed in monolayer epithelium or glandular epithelium of normal or tumor tissues. It is expressed in adenocarcinoma and the vast majority of nonkeratinizing squamous cell carcinoma, while keratinizing squamous cell carcinoma does not. It combined with CK5/6 for diagnosis adenocarcinoma and squamous cell carcinoma.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CK8/18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CK8/18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CK8/18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human keratinized oral squamous cell carcinoma. Anti-CK8/18 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon cancer. Anti-CK8/18 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti-CK8/18 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
