IHC shows positive staining in paraffin-embedded human tonsil. Anti-CK7/8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CK7/8 Recombinant Rabbit mAb (SDT-P006)
CK7/8 Recombinant Rabbit mAb (SDT-P006)
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Product Details
Product Details
Product Specification
| Synonyms | Keratin, type II cytoskeletal 7; Cytokeratin-7 (CK-7); Keratin-7 (K7); Sarcolectin; Type-II keratin Kb7; SCL; KRT7 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P08729、P05787 |
| Clone Number | SDT-P006 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
Cytokeratin 7/8 (CK7/8) proteins are intermediate filament proteins that play crucial roles in maintaining the structural integrity and function of epithelial cells. CK7, also known as sacolectin, is a type II cytokeratin typically found in glandular epithelia and transitional epithelium, including their associated tumors. CK8 is a type I cytokeratin that pairs with CK7 to form heterodimers, which are essential for the stability and function of the cytoskeleton in epithelial cells. These proteins are widely expressed in various organs and tissues, such as the thyroid, lung, female reproductive organs, upper digestive tract, and head and neck region. In pathology, CK7/8 are valuable markers for distinguishing epithelial tumors and identifying their origins. For example, CK7 is often positive in tumors of the breast, lung, and female reproductive system, while CK8 helps in further characterizing these tumors. Their expression patterns can also provide insights into the differentiation and behavior of tumor cells.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CK7/8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CK7/8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CK7/8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK7/8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
