IHC shows positive staining in paraffin-embedded human thymus. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CD278/ICOS Recombinant Rabbit mAb (SDT-R104)
CD278/ICOS Recombinant Rabbit mAb (SDT-R104)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD278/ICOS |
| Synonyms | Inducible T-cell costimulator, AILIM |
| Immunogen | N/A |
| Location | Secreted, Cell membrane |
| Accession | Q9Y6W8 |
| Clone Number | SDT-R104 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | null |
Background
Inducible T cell costimulatory factor (ICOS, cluster of differentiation, CD278) is an activated costimulatory immune checkpoint expressed on T cells. Its ligand ICOSL was expressed in antigen-presenting cells and somatic cells (including tumor cells in the tumor microenvironment) [PMID: 28713380, PMID: 12800259]. As one of the most common targeted immune checkpoints, ICOS is considered a possible way to develop new immunotherapy [PMID: 29544515]. Some clinical studies shown that blocking ICOS alone can inhibit tumor growth and proliferation, as well as TIGIT, CTLA4, and PD-1 [PMID: 30232519, PMID: 28803728].
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
