WB result of CA125 Rabbit mAb
Primary antibody: CA125 Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: OVCAR3 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate;
SK-OV-3 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 1519 kDa
CA125 Recombinant Rabbit mAb (SDT-555-41)
CA125 Recombinant Rabbit mAb (SDT-555-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CA125 |
| Synonyms | Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, MUC16, Cancer antigen 125 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane, Extracellular space |
| Accession | Q8WXI7 |
| Clone Number | SDT-555-41 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC | 1:500 |
Background
Mucin-16 (MUC-16), also known as ovarian cancer associated tumor marker CA125, is a protein encoded by the MUC16 gene. MUC-16 is a member of the glycoprotein family of mucins. MUC-16 has been applied as a tumor marker or biomarker, which may be elevated in the blood of certain types of cancer patients, especially those with cancer or other benign diseases. Detecting CA-125 blood levels is considered useful for treating ovarian cancer. Although this test can provide useful information for women who are known to have cancer, due to the uncertain correlation between CA-125 levels and cancer, the CA-125 test has not been found to be useful as a screening method. In addition to ovarian cancer, CA-125 also increases in patients with endometrial cancer, fallopian tube cancer, lung cancer, breast cancer, and gastrointestinal cancer. Pregnant women may also experience this condition in their bodies. Due to the fact that serum levels can be increased under various circumstances, CA-125 is not used for cancer detection, but it is typically used to monitor ovarian cancer patients' response to chemotherapy, recurrence, and disease progression.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
