WB result of BTK Recombinant Rabbit mAb
Primary antibody: BTK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Molt-4 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Lane 5: THP-1 whole cell lysate 20 µg
Negative control: Molt-4 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 70 kDa
BTK Recombinant Rabbit mAb (SDT-2105-37)
BTK Recombinant Rabbit mAb (SDT-2105-37)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BTK |
| Synonyms | Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase (ATK); B-cell progenitor kinase (BPK); Bruton tyrosine kinase; AGMX1; ATK; BPK; BTK |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | Q06187 |
| Clone Number | SDT-2105-37 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Raji, Daudi, Ramos, THP-1, RAW264.7, A20 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Bruton's tyrosine kinase (BTK) is a non-receptor tyrosine kinase that plays a crucial role in B-cell development and function. It is encoded by the BTK gene and consists of five distinct structural domains, including a kinase domain. BTK is essential for transmitting signals from B-cell antigen receptors, leading to processes like calcium mobilization and B-cell maturation. Mutations in the BTK gene can cause X-linked agammaglobulinemia (XLA), an immunodeficiency characterized by a lack of mature B cells and antibodies. In addition to its role in B cells, BTK is also involved in mast cell activation and has been implicated in various cancers, where its abnormal activation can lead to uncontrolled B-cell proliferation. Due to its involvement in B-cell signaling and cancer, BTK has become an important target for therapeutic interventions.
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Western Blot
WB result of BTK Recombinant Rabbit mAb
Primary antibody: BTK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 70 kDa
WB result of BTK Recombinant Rabbit mAb
Primary antibody: BTK Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-BTK antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-BTK antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-BTK antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-BTK antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-BTK antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-BTK antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
