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Human IL-22 ELISA Kit

Human IL-22 ELISA Kit

Catalog Number: abs510020 Application: ELISA Reactivity: Human Conjugation:
Price:
Regular price $450 USD
Regular price Sale price $450 USD
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Product Details

Product Specification

Synonym Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
Description

Testing Principle

This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human IL-22 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-22 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

 

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity100ul

Kit composition:

96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

Sensitivity:  1.8 pg / mL

Detection range:   31.3 - 2000 pg/mL

Recovery rate:  82-110%

Storage: 2-8 ° C

The standard curve:

Background: IL-22

Il-22 (interleukin-22) is a cytokine that induces ros, IL-6, IL-10, TNF -α, and neutrophil infiltration.It also supports the integrity of the epithelial barrier and induces epithelial cell proliferation during wound healing.The IL-22 signal is transmitted through a receptor complex consisting of IL-22R and IL-10R.Il-10 R beta is a common component of IL-10, IL-26, IL-28 and IL-29 receptor complexes.Il-22 binds to IL-22BP and blocks the interaction between IL-22 and IL-22R.

Picture

Immunohistochemistry

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