1μg (R: reducing condition, N: non-reducing condition).
NT-3 Protein, Human
NT-3 Protein, Human
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$197 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,NTF3 |
| Accession | P20783 |
| Amino Acid Sequence | Tyr139-Thr257YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT |
| Expression System | E.coli |
| Molecular Weight | 15kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 500mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
·12 months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles. |
| Reference |
1. Elizabeth Hernández-Echeagaray (2020) Vitam Hor. 114:71-89. 2. F Marmigère. (2001) Neuroendocrinology 74(1):43-54. |
Background
Neurotrophin-3 (NT-3) belongs to a family of growth factors called neurotrophins whose actions are centered in the nervous system. The neurotrophin family includes nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-4/5 (NT-4/5), neurotrophin-6(NT-6) and the recently cloned neurotrophin-7 Most of biological effects of neurotrophins are mediated by high affinity tyrosine kinase (Trk) receptors, although some of them require the binding to a low affini tyreceptor (p75LNTR). NGF binds to TrkA receptors, BDNF preferentially activates TrkB receptors, while NT-3 mainly interacts with TrkC receptors, and at lower affinity with TrkB receptors.NT-3 is structurally related to other neurotrophins like brain-derived neurotrophic factor. The expression of NT-3 starts with the onset of neurogenesis and continues throughout life. A wealth of information links NT-3 to the growth, differentiation, and survival of hippocampal cells as well as sympathetic and sensory neurons.
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SDS-PAGE
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human NT-3 is immobilized 1µg/mL (100 µl/well), Recombinant Human TrκB Fc, Human binds with an EC50 of 0.015-0.02μg/ml.
Immobilized NT-3 Protein, Human(Cat. No. UA040216) at 1.0μg/mL (100μL/well) can bind TrkC Fc Chimera Protein, Human (UA010243) with EC50 of 7.48-18.2 ng/mL.
