Determined by the dose-dependent stimulation of the proliferation of human OCI-AML5 cells.The EC50 for this effect is less than 3ng/ml.
FLT-3L Protein, Mouse
FLT-3L Protein, Mouse
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$204 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine |
| Accession | P49772 |
| Amino Acid Sequence | Gly27-Arg188, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 20-33kDa(Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Cancer Res. 2009 Oct 1,69(19):7747-55. 2.Blood, Jul 2009, 114: 835 - 843. 3. J. Immunol., Jun 2009, 182: 7408 - 7414. |
Background
FLT-3 ligand promotes long- term expansion anddifferentiation of human pro-B-cells in the presence of IL-7 or in combinationof IL-7 and IL-3.The human FLT-3 ligand also stimulates the proliferation ofcells expressing murine FLT-3 receptors. In combination with SCF and IL-3,FLT-3 ligand can cause expansion of cells with the marker spectrum CD34(+)CD38(-). Alone, FLT-3 ligand supports the survival of precursors in the lineageof blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive highproliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only hasmarginal effects on progenitors for erythroid cells and megakaryocytes.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized FLT-3L Protein, Mouse (Cat. No. UA040159) at 1.0μg/mL (100μL/well) can bind Flt-3/Flk-2 Protein, Human (UA040316) with EC50 of 2.11-3.21 ng/mL.
