Standard curve
Example of IL-17A standard curve in Assay Diluent .
Human IL-17A Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-17A Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-17A |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.71%； Inter-assay: 2.76% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.22 pg/mL |
| Range | 1.87 - 8000 pg/mL |
| Recovery | 74.32% - 104.91% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As IL-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.
Picture
Picture
CBA
Recovery
Within the detection range, the human IL-17A protein was added to different matrices at high, medium, low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5 μg/mL PHA for 24h, and the average concentration of IL-17A in the supernatant was measured to be 321.51 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-17A in the supernatant was measured to be 0.29 pg/mL.
Linearity
The high-concentration human IL-17A protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
Then, the concentration of human IL-17A was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
