Standard curve
Example of IL-1β standard curve in Assay Diluent .
Human IL-1β Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-1β Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-1β |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.83%； Inter-assay: 6.45% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.06 pg/mL |
| Range | 1.19 - 5000 pg/mL |
| Recovery | 91.64% - 109.52% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1βgene. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as Cryopyrin-Associated Periodic Syndromes (CAPS), due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β.
Picture
Picture
CBA
Recovery
Within the detection range, the human IL-1β protein was added to different matrices at high, medium, low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5 μg/mL PHA for 24h, and the average concentration of IL-1β in the supernatant was measured to be 2104.39 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-1β in the supernatant was measured to be 520.92 pg/mL.
Linearity
The high-concentration human IL-1β protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
Then, the concentration of human IL-1β was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
