Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) labelling Human CD294 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Rat IgG2a, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD123 - FITC antibody separately.
Rat Anti-Human CD294 Antibody (S-2867)
Rat Anti-Human CD294 Antibody (S-2867)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD294 |
| Synonyms | Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CRTH2; DL1R; GPR44; PTGDR2 |
| Location | Cell membrane |
| Accession | Q9Y5Y4 |
| Clone Number | S-2867 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:200 | Hu |
Background
CD294, also known as CRTH2, PTGDR2, or GPR44, is a seven-transmembrane G protein-coupled receptor primarily expressed on Th2 cells, eosinophils, basophils, and type-2 innate lymphoid cells (ILC2). It is involved in mediating the chemotaxis of these cells to sites of allergic inflammation, such as the asthmatic lung, and plays a role in preventing apoptosis in Th2 cells.
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