Flow cytometric analysis of Human CD235a expression on Human Peripheral Blood cells. Human Peripheral Blood cells were stained with PerCP-Cy5.5 Mouse IgG2a, κ Isotype Control (Black line histogram) or PerCP-Cy5.5 Mouse Anti-Human CD235a Antibody (Red line histogram) at 1.25 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Percp-Cy5.5 Mouse Anti-Human CD235a Antibody (S-R473)
Percp-Cy5.5 Mouse Anti-Human CD235a Antibody (S-R473)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD235a |
| Synonyms | Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; GPA; MNS; GYPA |
| Location | Cell membrane |
| Accession | P02724 |
| Clone Number | S-R473 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human Peripheral Blood cells |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD235a protein, also known as Glycophorin A, is a major intrinsic membrane protein of human red blood cells and erythroid precursor cells. It is a type I sialoglycoprotein with a molecular weight of around 10 kD, present in the cell membrane as a homodimer. This protein serves as a carrier for the MN blood group antigens. It also plays a crucial role in maintaining the integrity and shape of the erythrocyte membrane by being a part of the ankyrin-1 complex. Additionally, CD235a acts as a receptor for several pathogens, including the influenza virus, Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175), and hepatitis A virus.
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