Flow cytometric analysis of IL-17A expression in Th17 polarized C57BL/6 mouse splenocytes. Th17 polarized and C57BL/6 mouse splenocytes stimulated 6 hours with 500ng/ml PMA and 500ng/ml Ionomycin in the presence of 300ng/ml BFA (Right Panel) or untreated Th17 polarized C57BL/6 mouse splenocytes (Left Panel) were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with FITC Rat anti-Mouse CD4 antibody (S0B1646) and SDT PE Rat Anti-Mouse IL-17A Antibody at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Rat Anti-Mouse IL-17A Antibody (S-R683)
PE Rat Anti-Mouse IL-17A Antibody (S-R683)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-17A |
| Synonyms | Interleukin-17A; IL-17; Cytotoxic T-lymphocyte-associated antigen 8 (CTLA-8); Ctla8; Il17; Il17a |
| Location | Secreted |
| Accession | Q62386 |
| Clone Number | S-R683 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | Th17 polarized C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
IL-17A protein a is key cytokine produced by various immune cells such as Th17 cells. It plays a crucial role in the immune response by promoting inflammation. It can induce the expression of various pro-inflammatory molecules in different cell types, including chemokines that recruit neutrophils and other immune cells to the site of infection or injury. IL-17A is involved in the defense against certain pathogens, but its excessive production is also associated with the pathogenesis of several autoimmune and inflammatory diseases like psoriasis and rheumatoid arthritis. Targeting IL-17A has become an important therapeutic strategy in the treatment of these conditions.
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