Flow cytometric analysis of Mouse CD45RA expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 and either PE Rat IgG2b, κ Isotype Control (Left panel) or SDT PE Rat Anti-Mouse CD45RA Antibody (Right panel) at 5 μl/test. Total viable cells, as determined by SDT Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Rat Anti-Mouse CD45RA Antibody (S-R564)
PE Rat Anti-Mouse CD45RA Antibody (S-R564)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD45RA |
| Synonyms | Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc |
| Location | Cell membrane, Synapse |
| Accession | P06800 |
| Clone Number | S-R564 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD45RA is an isoform of the CD45 protein, a member of the protein tyrosine phosphatase (PTP) family, and serves as a critical regulator of T- and B-cell antigen receptor signaling. It is predominantly expressed on the surface of B cells, from early pro-B cells to mature and activated B cells, making it a commonly used pan-B cell marker in mice. Additionally, CD45RA is also found on certain subsets of activated T cells, lymphokine-activated killer cells (LAK), NK cell progenitors, and T cells in mutant mice. This molecule plays a crucial role in lymphocyte development and antigen signaling, and its expression is regulated during different stages of B cell maturation.
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