Flow cytometric analysis of mouse CD185 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either Phycoerythrin Rat IgG2b, κ Isotype Control (left panel) or SDT Phycoerythrin Rat Anti-Mouse CD185 Antibody (right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Rat Anti-Mouse CD185 Antibody (S-R600)
PE Rat Anti-Mouse CD185 Antibody (S-R600)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD185 |
| Synonyms | C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1 homolog; Blr1; Gpcr6; Cxcr5 |
| Location | Cell membrane |
| Accession | Q04683 |
| Clone Number | S-R600 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 0.25μg per million cells in 100μl volume | Ms |
Background
CD185, also known as CXCR5 or Burkitt lymphoma receptor 1 (BLR1), is a G protein-coupled receptor with seven transmembrane regions. It belongs to the CXC chemokine receptor family and specifically binds to the chemokine CXCL13. This protein is crucial for the migration of B cells into B cell follicles in the spleen and Peyer's patches, and it is also essential for the function of follicular helper T cells. CD185 is expressed in mature B cells, follicular helper T cells, and certain types of lymphoma, including Burkitt's lymphoma. Additionally, it may play a role in maintaining cardiac structure under pressure overload and in the formation of ectopic mucosa-associated lymphoid tissue during chronic inflammation.
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