Human PBMC were stained with PE Mouse IgG2b,κ Isotype Control at 1/20 dilution (1 μg). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
PE Mouse IgG2b, κ Isotype Control Antibody (S-844-22)
PE Mouse IgG2b, κ Isotype Control Antibody (S-844-22)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-22 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of 4% paraformaldehyde fixed and 0.1% Tween permeabilized human PBMC labeled with PE Mouse IgG2b,κ Isotype Control at 1/20 dilution (1 μg). Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
