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PE-Cy7 Mouse anti-Human CD90 Antibody (5E10)

PE-Cy7 Mouse anti-Human CD90 Antibody (5E10)

Catalog Number: S0B81058 Application: FCM Reactivity: Hu Conjugation: PE-Cy7 Brand: Starter
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Regular price $190 USD
Regular price Sale price $190 USD
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Product Details

Product Specification


Host Mouse
Antigen CD90
Location Cell membrane
Accession P04216
Clone Number 5E10
Antibody Type Mouse mAb
Isotype IgG1
Isotype Control S0B80046
Application FCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein G
Concentration 0.2 mg/ml
Conjugation PE-Cy7
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

Picture

FC

Flow cytometric analysis of CD90 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with Alexa Fluor® 647 Mouse Anti-Human CD34 Antibody and either PE-Cy7 Mouse IgG1 Isotype Control (left panel) or SDT PE-Cy7 Mouse Anti-Human CD90 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.