Flow cytometric analysis of IL-9 expression in human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (Right Panel) or unstimulated (Left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT Pacific Blue Mouse Anti-Human IL-9 Antibody at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software."
Pacific Blue Mouse Anti-Human IL-9 Antibody (S-2920)
Pacific Blue Mouse Anti-Human IL-9 Antibody (S-2920)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | IL-9 |
| Synonyms | Interleukin-9; Cytokine P40; T-cell growth factor P40; IL9 |
| Location | Secreted |
| Accession | P15248 |
| Clone Number | S-2920 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Th2 polarized human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Background
Interleukin-9 (IL-9) is a 14 kDa glycoprotein cytokine primarily produced by activated Th2, Th9, and innate lymphoid cells that signals through a heterodimeric receptor composed of the IL-9Rα chain and the common γ-chain, thereby activating JAK/STAT, PI3K/AKT, and MAPK pathways to exert pleiotropic effects on hematopoietic and epithelial cells, promoting mast-cell proliferation and differentiation, enhancing mucus production by goblet cells, modulating B-cell responses, and contributing to the pathogenesis of allergic asthma, atopic dermatitis, and certain hematologic malignancies, while also exerting context-dependent anti-tumor or pro-tumor activities depending on the cellular milieu.
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