Flow cytometric analysis of Human CD4 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 antibody and either Pacific Blue Mouse IgG1 Isotype Control (Left panel) or SDT Pacific Blue Mouse Anti-Human CD4 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Mouse Anti-Human CD4 Antibody (S-574-16)
Pacific Blue Mouse Anti-Human CD4 Antibody (S-574-16)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD4 |
| Synonyms | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01730 |
| Clone Number | S-574-16 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD4 is a glycoprotein found on the surface of certain immune cells, such as helper T cells, monocytes, macrophages, and dendritic cells. It is a monomeric type I transmembrane molecule composed of four immunoglobulin-like domains (D1 to D4) and encoded by a single gene. As a co-receptor for the T-cell receptor (TCR), CD4 plays a crucial role in the immune response by binding to the MHC class II molecules on antigen-presenting cells, which helps T cells recognize and respond to foreign antigens. The interaction between CD4 and MHC class II molecules also activates downstream signaling pathways, leading to the activation of transcription factors and promoting T cell activation. Additionally, CD4 is significant in HIV infection, as the virus binds to CD4 to enter host cells.
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