Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labeling Mouse IgG2c Isotype Control antibody at 1/200 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Mouse IgG2c Isotype Control (S-4245)
Mouse IgG2c Isotype Control (S-4245)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-4245 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2c,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized human PBMC (human peripheral blood mononuclear cells) labelling mouse IgG2c Isotype Control antibody at 1/200 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
