Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized PBMC (Human peripheral blood mononuclear cells) labelling Granzyme B antibody at 1/200 dilution (1 μg)/ (Right panel) compared with a Mouse IgG1, κ Isotype Control (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8 - Brilliant Violet 421™ antibody separately.
Mouse Anti-Human Granzyme A Antibody (S-3175)
Mouse Anti-Human Granzyme A Antibody (S-3175)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Granzyme A |
| Synonyms | CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor (H factor; HF); CTLA3; HFSP; GZMA |
| Location | Secreted |
| Accession | P12544 |
| Clone Number | S-3175 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Hu |
Background
Granzyme A (GZMA) is a 28 kDa serine protease stored in the cytotoxic granules of CD8⁺ T cells and NK cells; upon immunological synapse formation, it enters target cells together with perforin and cleaves after Lys or Arg residues to hydrolyze SET complex proteins (including the SET, APE1, and HMGB2 nucleases), thereby disrupting DNA repair and initiating a caspase-independent, oxidative DNA-damage cell death pathway that manifests as single-stranded DNA nicks, mitochondrial dysfunction, and plasma membrane perforation, while extracellularly it can process extracellular matrix components and cytokine precursors to modulate inflammation, vascular integrity, and antiviral immunity.
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