Flow cytometric analysis of Human IgM expression on Human Peripheral Blood cells. Human Peripheral Blood cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 Antibody and either Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human IgM Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human IgM Antibody (S-3031)
FITC Mouse Anti-Human IgM Antibody (S-3031)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Human IgM |
| Synonyms | Immunoglobulin heavy constant mu; Ig mu chain C region; Ig mu chain C region BOT; Ig mu chain C region GAL; Ig mu chain C region OU; IGHM |
| Location | Secreted, Cell membrane |
| Accession | P01871 |
| Clone Number | S-3031 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human Peripheral Blood cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
Human IgM is the first type of antibody produced by the body in response to an initial exposure to an antigen. It is a large, pentameric molecule, composed of five identical Y-shaped units linked together. Each IgM molecule has ten antigen-binding sites, which allows it to be highly effective at agglutinating pathogens. IgM is primarily found in the bloodstream and plays a crucial role in the early stages of the immune response. It can activate the complement system, enhancing the ability to eliminate pathogens. Additionally, the presence of IgM antibodies in diagnostic tests often indicates a recent infection, as it appears earlier than other types of antibodies like IgG.
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