Multiparameter flow cytometric analysis of Human CD8α expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD4 antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD8α Antibody (Right panel) at 0.5 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human CD8α Antibody (S-1036-34)
FITC Mouse Anti-Human CD8α Antibody (S-1036-34)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD8α |
| Synonyms | T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain, MAL, CD8a |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01732 |
| Clone Number | S-1036-34 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 0.1mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
CD8A encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
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