Flow cytometric analysis of mouse splenocytes labeling Biotin Rat IgG2b Isotype Control at 1/20 dilution (1 μg) / (Right panel) followed by Sav-iFluor 488 compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat IgG2b Isotype Control (S-R486)
Biotin Rat IgG2b Isotype Control (S-R486)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-R486 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b |
| Application | FCM, ICFCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeling Biotin Rat IgG2b Isotype Control at 1/20 dilution (1 μg) followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
